구강 편평상피세포암의 골전이 모델
Bone Metastasis Model of Oral Squamous Cell Carcinoma
박영욱, 오유진, 이희수,
소속 상세정보
박영욱 ( Park Young-Wook ) - 강릉원주대학교 치과대학 구강악안면외과학교실
오유진 ( Oh Yu-jin ) - 강릉원주대학교 치과대학 구강악안면외과학교실
이희수 ( Lee Hee-Su ) - 강릉원주대학교 치과대학 구강해부학교실
KMID : 0360120100320020118
Abstract
Background and Purpose: Bone metastases rarely occur in patients with oral squamous cell carcinoma (OSCC), so the molecular mechanisms of bone metastasis of OSCC remains unclear. Studies with animal models allow progresses in understanding the molecular events for bone metastasis and provide new targets for therapy. So we tried to establish a murine model for bone metastasis of oral squamous cell carcinoma.
Materials and Methods: Human OSCC cells (KB cell line) were xenografted to nude mice via direct inoculation into the tibial marrow. Mice with tibial tumors were sacrificed once a week, until seven weeks after the injection of human tumor cells. Growth of tibial tumors were observed by histology. Expression of TGF-beta and CXCR-4 in bone OSCC (experimental) and subcutaneous tumor (control) was also evaluated by immunohistochemical staining.
Results: Bone OSCC was successfully induced by intra-tibial injection of KB cells. Tumor mass was developed in the marrow tissues of tibia and finally invade the endosteum of tibia. Immunohistochemical staining showed higher expression of TGF-beta in bone tumors than in subcutaneous tumors.
Conclusion: A murine model of bone metastasis of OSCC was suggested that imitated the clinical findings of distant vascular metastasis. This bone tumor model should facilitate understanding of the molecular pathogenesis of OSCC bone metastasis, and aid in the developement of treatment strategies against OSCC bone metastasis.
키워드
Oral squamous cell carcinoma;Bone metastasis;Animal model
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